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Image Search Results
Journal: PLoS ONE
Article Title: Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells
doi: 10.1371/journal.pone.0119189
Figure Lengend Snippet: Monolayers of BSC1 and RK13 cells were initially infected with a low amount of ECTV-GFP (MOI = 0.001). On day 5 post-infection, the monolayers were examined for the formation of plaques. As expected, ECTV formed plaques on BSC1 cells (a representative example is shown on the bottom row). However, no plaque formation was observed on RK13 cells (a representative example is shown on the top row). All images are at 100x total magnification.
Article Snippet: The following viruses were used during the course of this work: wild-type ECTV (Moscow strain), ECTV (Moscow strain) expressing GFP [ ], VACV (Western Reserve strain) expressing
Techniques: Infection
Journal: PLoS ONE
Article Title: Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells
doi: 10.1371/journal.pone.0119189
Figure Lengend Snippet: (A) VACVΔE3LΔK3L was constructed using homologous recombination techniques and engineered to express GFP in place of the E3L gene. No plaque formation was visualized in wild-type RK13 cells whereas replication capacity was restored in RK13 cells stably expressing E3 and K3 proteins derived from VACV. Representative images are shown. (B) The total yield of VACV wild-type, VACVΔE3L, VACVΔK3L, and VACVΔE3LΔK3L was determined using wild-type RK13 (red bars) and RK13+E3L+K3L (gray bars) cells. Cells were initially infected with a low amount of virus (MOI = 0.01). Virus was harvested 30 hours post-infection and the total yield was determined using standard plaque assays with RK13+E3L+K3L cells. The data is the average of two independent experiments with error bars representing the standard deviation. P-values were determined in Microsoft Excel using the Student’s t-test.
Article Snippet: The following viruses were used during the course of this work: wild-type ECTV (Moscow strain), ECTV (Moscow strain) expressing GFP [ ], VACV (Western Reserve strain) expressing
Techniques: Construct, Homologous Recombination, Stable Transfection, Expressing, Derivative Assay, Infection, Virus, Standard Deviation
Journal: PLoS ONE
Article Title: Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells
doi: 10.1371/journal.pone.0119189
Figure Lengend Snippet: BSC1 (black bars), RK13 (red bars), RK13-E3L (blue bars), and RK13-E3L+K3L (gray bars) cells were initially infected with a low amount of VACV-GFP (MOI = 0.01) (A and B) or ECTV-GFP (MOI = 0.01) (C and D) . Spread of virus in the culture well was quantified using flow cytometry. The histograms (A and C) depict relative fluorescence intensity among total cells at day 3 post-infection. The bar graphs (B and D) quantify levels of GFP on days 1, 2, and 3 post-infection in each cell type. The bars depict the average values and the error bars represent the standard deviations of three independent trials. The yield of w.t. VACV (E) and ECTV (F) was determined 48 hours after initial infection with a high amount of virus (MOI = 5) on cell monolayers. For quantification using a standard plaque assay, harvested virus was added to monolayers of BSC1 cells after serial dilution. The bars depict the average values and the error bars represent the standard deviations of three independent trials. Statistical analysis [performed using GraphPad Prism software (version 5.0a)] was carried out using a one-way ANOVA (nonparametric; Kruskal-Wallis) followed by a Dunns test for multiple comparisons. * denotes a p value <0.05.
Article Snippet: The following viruses were used during the course of this work: wild-type ECTV (Moscow strain), ECTV (Moscow strain) expressing GFP [ ], VACV (Western Reserve strain) expressing
Techniques: Infection, Virus, Flow Cytometry, Fluorescence, Plaque Assay, Serial Dilution, Software