type western reserve strain Search Results


90
BEI Resources western reserve (wr) vaccinia strain
Western Reserve (Wr) Vaccinia Strain, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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China Center for Type Culture Collection vacv western reserve strain
Vacv Western Reserve Strain, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gloeckner Foundation recombinant vacv strain western reserve (wr)
Recombinant Vacv Strain Western Reserve (Wr), supplied by Gloeckner Foundation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BEI Resources vacv (western reserve strain) expressing gfp
Monolayers of BSC1 and RK13 cells were initially infected with a low amount <t>of</t> <t>ECTV-GFP</t> (MOI = 0.001). On day 5 post-infection, the monolayers were examined for the formation of plaques. As expected, ECTV formed plaques on BSC1 cells (a representative example is shown on the bottom row). However, no plaque formation was observed on RK13 cells (a representative example is shown on the top row). All images are at 100x total magnification.
Vacv (Western Reserve Strain) Expressing Gfp, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
vacv (western reserve strain) expressing gfp - by Bioz Stars, 2026-05
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Imanis Life Sciences LLC vaccinia virus, western reserve strain, encoding a bacteriophage t7 rna polymerase transgene
Monolayers of BSC1 and RK13 cells were initially infected with a low amount <t>of</t> <t>ECTV-GFP</t> (MOI = 0.001). On day 5 post-infection, the monolayers were examined for the formation of plaques. As expected, ECTV formed plaques on BSC1 cells (a representative example is shown on the bottom row). However, no plaque formation was observed on RK13 cells (a representative example is shown on the top row). All images are at 100x total magnification.
Vaccinia Virus, Western Reserve Strain, Encoding A Bacteriophage T7 Rna Polymerase Transgene, supplied by Imanis Life Sciences LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vaccinia virus, western reserve strain, encoding a bacteriophage t7 rna polymerase transgene/product/Imanis Life Sciences LLC
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vaccinia virus, western reserve strain, encoding a bacteriophage t7 rna polymerase transgene - by Bioz Stars, 2026-05
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SmithKline Beecham Clinical vv wild strain
Monolayers of BSC1 and RK13 cells were initially infected with a low amount <t>of</t> <t>ECTV-GFP</t> (MOI = 0.001). On day 5 post-infection, the monolayers were examined for the formation of plaques. As expected, ECTV formed plaques on BSC1 cells (a representative example is shown on the bottom row). However, no plaque formation was observed on RK13 cells (a representative example is shown on the top row). All images are at 100x total magnification.
Vv Wild Strain, supplied by SmithKline Beecham Clinical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Bavarian Nordic vaccinia virus western reserve strain vv wr prnt assay
Monolayers of BSC1 and RK13 cells were initially infected with a low amount <t>of</t> <t>ECTV-GFP</t> (MOI = 0.001). On day 5 post-infection, the monolayers were examined for the formation of plaques. As expected, ECTV formed plaques on BSC1 cells (a representative example is shown on the bottom row). However, no plaque formation was observed on RK13 cells (a representative example is shown on the top row). All images are at 100x total magnification.
Vaccinia Virus Western Reserve Strain Vv Wr Prnt Assay, supplied by Bavarian Nordic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Monolayers of BSC1 and RK13 cells were initially infected with a low amount of ECTV-GFP (MOI = 0.001). On day 5 post-infection, the monolayers were examined for the formation of plaques. As expected, ECTV formed plaques on BSC1 cells (a representative example is shown on the bottom row). However, no plaque formation was observed on RK13 cells (a representative example is shown on the top row). All images are at 100x total magnification.

Journal: PLoS ONE

Article Title: Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells

doi: 10.1371/journal.pone.0119189

Figure Lengend Snippet: Monolayers of BSC1 and RK13 cells were initially infected with a low amount of ECTV-GFP (MOI = 0.001). On day 5 post-infection, the monolayers were examined for the formation of plaques. As expected, ECTV formed plaques on BSC1 cells (a representative example is shown on the bottom row). However, no plaque formation was observed on RK13 cells (a representative example is shown on the top row). All images are at 100x total magnification.

Article Snippet: The following viruses were used during the course of this work: wild-type ECTV (Moscow strain), ECTV (Moscow strain) expressing GFP [ ], VACV (Western Reserve strain) expressing GFP (BEI resources NR-624), wild-type VACV (Copenhagen strain), VACVΔE3L (Copenhagen background), VACVΔK3L (Copenhagen background), and VACVΔE3LΔK3L (Copenhagen background).

Techniques: Infection

(A) VACVΔE3LΔK3L was constructed using homologous recombination techniques and engineered to express GFP in place of the E3L gene. No plaque formation was visualized in wild-type RK13 cells whereas replication capacity was restored in RK13 cells stably expressing E3 and K3 proteins derived from VACV. Representative images are shown. (B) The total yield of VACV wild-type, VACVΔE3L, VACVΔK3L, and VACVΔE3LΔK3L was determined using wild-type RK13 (red bars) and RK13+E3L+K3L (gray bars) cells. Cells were initially infected with a low amount of virus (MOI = 0.01). Virus was harvested 30 hours post-infection and the total yield was determined using standard plaque assays with RK13+E3L+K3L cells. The data is the average of two independent experiments with error bars representing the standard deviation. P-values were determined in Microsoft Excel using the Student’s t-test.

Journal: PLoS ONE

Article Title: Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells

doi: 10.1371/journal.pone.0119189

Figure Lengend Snippet: (A) VACVΔE3LΔK3L was constructed using homologous recombination techniques and engineered to express GFP in place of the E3L gene. No plaque formation was visualized in wild-type RK13 cells whereas replication capacity was restored in RK13 cells stably expressing E3 and K3 proteins derived from VACV. Representative images are shown. (B) The total yield of VACV wild-type, VACVΔE3L, VACVΔK3L, and VACVΔE3LΔK3L was determined using wild-type RK13 (red bars) and RK13+E3L+K3L (gray bars) cells. Cells were initially infected with a low amount of virus (MOI = 0.01). Virus was harvested 30 hours post-infection and the total yield was determined using standard plaque assays with RK13+E3L+K3L cells. The data is the average of two independent experiments with error bars representing the standard deviation. P-values were determined in Microsoft Excel using the Student’s t-test.

Article Snippet: The following viruses were used during the course of this work: wild-type ECTV (Moscow strain), ECTV (Moscow strain) expressing GFP [ ], VACV (Western Reserve strain) expressing GFP (BEI resources NR-624), wild-type VACV (Copenhagen strain), VACVΔE3L (Copenhagen background), VACVΔK3L (Copenhagen background), and VACVΔE3LΔK3L (Copenhagen background).

Techniques: Construct, Homologous Recombination, Stable Transfection, Expressing, Derivative Assay, Infection, Virus, Standard Deviation

BSC1 (black bars), RK13 (red bars), RK13-E3L (blue bars), and RK13-E3L+K3L (gray bars) cells were initially infected with a low amount of VACV-GFP (MOI = 0.01) (A and B) or ECTV-GFP (MOI = 0.01) (C and D) . Spread of virus in the culture well was quantified using flow cytometry. The histograms (A and C) depict relative fluorescence intensity among total cells at day 3 post-infection. The bar graphs (B and D) quantify levels of GFP on days 1, 2, and 3 post-infection in each cell type. The bars depict the average values and the error bars represent the standard deviations of three independent trials. The yield of w.t. VACV (E) and ECTV (F) was determined 48 hours after initial infection with a high amount of virus (MOI = 5) on cell monolayers. For quantification using a standard plaque assay, harvested virus was added to monolayers of BSC1 cells after serial dilution. The bars depict the average values and the error bars represent the standard deviations of three independent trials. Statistical analysis [performed using GraphPad Prism software (version 5.0a)] was carried out using a one-way ANOVA (nonparametric; Kruskal-Wallis) followed by a Dunns test for multiple comparisons. * denotes a p value <0.05.

Journal: PLoS ONE

Article Title: Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells

doi: 10.1371/journal.pone.0119189

Figure Lengend Snippet: BSC1 (black bars), RK13 (red bars), RK13-E3L (blue bars), and RK13-E3L+K3L (gray bars) cells were initially infected with a low amount of VACV-GFP (MOI = 0.01) (A and B) or ECTV-GFP (MOI = 0.01) (C and D) . Spread of virus in the culture well was quantified using flow cytometry. The histograms (A and C) depict relative fluorescence intensity among total cells at day 3 post-infection. The bar graphs (B and D) quantify levels of GFP on days 1, 2, and 3 post-infection in each cell type. The bars depict the average values and the error bars represent the standard deviations of three independent trials. The yield of w.t. VACV (E) and ECTV (F) was determined 48 hours after initial infection with a high amount of virus (MOI = 5) on cell monolayers. For quantification using a standard plaque assay, harvested virus was added to monolayers of BSC1 cells after serial dilution. The bars depict the average values and the error bars represent the standard deviations of three independent trials. Statistical analysis [performed using GraphPad Prism software (version 5.0a)] was carried out using a one-way ANOVA (nonparametric; Kruskal-Wallis) followed by a Dunns test for multiple comparisons. * denotes a p value <0.05.

Article Snippet: The following viruses were used during the course of this work: wild-type ECTV (Moscow strain), ECTV (Moscow strain) expressing GFP [ ], VACV (Western Reserve strain) expressing GFP (BEI resources NR-624), wild-type VACV (Copenhagen strain), VACVΔE3L (Copenhagen background), VACVΔK3L (Copenhagen background), and VACVΔE3LΔK3L (Copenhagen background).

Techniques: Infection, Virus, Flow Cytometry, Fluorescence, Plaque Assay, Serial Dilution, Software